Journal: Journal of Advanced Research
Article Title: Fish oil reduces intestinal fat absorption by promoting lacteal junction zippering via GPR120-VEGFR3-MLCK pathway
doi: 10.1016/j.jare.2025.05.026
Figure Lengend Snippet: Inhibition of MLCK signaling mimicked the effects of DHA and EPA on the proportion of zipper-like junctions in PA-treated LECs. (A-D) Effects of ML-7 (50 μM) treatment on the proportion of zipper-like junction proportion (A, B) and the expression of MLCK, p-MLC2 (C, D) in PA-treated (100 μM) LECs. White dashed line: VE-cadherin-negative junctions; white arrow: button-like junctions; yellow arrow: zipper-like junctions. Two-tailed unpaired Student’s t -test was performed to compare PA versus PA + DHA/EPA/ML-7-treated groups at the same time points. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)
Article Snippet: Primary antibodies include CD36 (proteintech,18836–1-AP), VEGFC (proteintech,67116–1-Ig), VEGFR3 (R&D, AF743), ApoB48 (ThermoFisher, MA5-35458), MTTP (ThermoFisher, PA5-102474,), MLCK (ThermoFisher Scientific, PA5100908), GPR120 (Santa Cruz, sc-9901), p-MLC2 (CST, 95777S, Thr18/Ser19), GAPDH (Bioworld, AP0063), β-actin (Bioworld, AP0060) and β-Tubulin (Bioworld, AP0064).
Techniques: Inhibition, Expressing, Two Tailed Test